Asia Pacific Journal of Molecular Biology and Biotechnology (APJMBB) is an open accessed journal publishing research findings in the fields of Biotechnology and Molecular Biology. The Journal aims to promote research in all relevant areas of molecular & cellular biology in prokaryotic & eukaryotic (including methodology) and biotechnology (microbial, agricultural, animal, forensic, aquatic, medical, bioremediation, and regulatory biotechnology) in the Asia Pacific region through publication of research articles, both basic and applied.
|3rd International Conference on Molecular Biology and Biotechnology 2019|
|The 3rd International Conference on Molecular Biology and Biotechnology was held on 24-25 April 2019 in UCSI University, Kuala Lumpur, Malaysia. This supplementary comprises of all abstracts presented during the conference in the form of oral, pecha kucha, and poster presentation.|
|Download APJMBB 27(2) Suppl.|
Evaluation of acute and sub-acute oral toxicity of the aqueous extract of Aquilaria malaccensis leaves in Sprague Dawley rats
|Redzuan Nul Hakim Abdul Razak, Suzanah Abdul Rahman, Asmah Hanim Hamdan, Roszaman Ramli, Muhammad Lokman Md. Isa, Hussin Muhammad, Nik Fakhuruddin Nik Hassan|
|Aquilaria malaccensis or commonly known as ‘gaharu’ is a species of Aquilaria genus and belongs to the Thymelaeaceae family. It is widely distributed in Malaysia, Indonesia, and the Borneo Islands. Traditionally, its leaves were used to relieve bruises and studies have shown that they function as an antioxidant, aphrodisiac, and tranquilizer. Despite its proven beneficial medicinal properties, information regarding its toxicity is limited. Therefore, we performed a safety evaluation on the aqueous A. malaccensis leaves extract (AMAE) in Sprague Dawley rats. The assessment of acute toxicity based on the Organization for Economic Cooperation and Development (OECD) Guideline 420 revealed that AMAE did not influence mortality, clinical appearance, body weight gain, or necropsy findings at a dose of 2000 mg/kg body weight. In the sub-acute toxicity, all doses did not significantly modify the body weight and food and water intake. In male rats treated with 2000 mg/kg, there was a significant reduction in the relative weight of liver. Not only that, an increase in alkaline phosphatase and alanine transaminase was also observed in different groups among the female rats. A significant decrease in the creatinine level was also seen among male rats administered with different doses of AMAE. In both sexes, histopathological analysis had shown abnormalities in the liver and kidney of rats treated at the dose of 2000 mg/kg. In conclusion, the 50% lethal dose (LD50) of AMAE was estimated to be greater than 2000 mg/kg. In sub-acute duration, the findings suggested that AMAE administered orally is slightly toxic at higher doses (2000 mg/kg) and could provoke functional and structural changes in the kidney and liver of rats. Thus, the extract should be used with caution.
(A) Photomicrograph of kidney section of a control rat in subacute toxicity study of AMAE sacrificed at the end of study period showing normal morphology of glomerulus, tubular epithelial cells and Bowman’s space (H&E stain, X20). (B) Photomicrograph of kidney section of a rat AMAE 20 in subacute toxicity of AMAE sacrificed at the end of study period showing no abnormality (H&E stain, X40). (C) Photomicrograph of kidney section of a rat AMAE 200 in subacute toxicity study of AMAE sacrificed at the end of study period showing intact cellular arrangements and histology (H&E stain, X40). (D) Photomicrograph of kidney section of a rat AMAE 2000 in subacute toxicity study of AMAE sacrificed at the end of study period showing cytoplasmic vacuolation and appearance of pyknotic nuclei (H&E stain, X40). B: Bowman’s space, BC: Bowman’s capsule, G: Glomerulus, PCT: Proximal convoluted tubule, EC: Tubular epithelial cell, CV: Cytoplasmic vacuolation, PN: Pyknotic nuclei.